Properties of Proteus mirabilis phage 13vir
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چکیده
منابع مشابه
Derivation of a Proteus mirabilis converting phage for ampicillin resistance.
A lysate of phage 5006M was prepared on Proteus mirabilis ~ ~ 5 0 0 6 which harboured the plasmid P-lac to which the ampicillin resistance gene of R plasmid RP4 had been transposed. This lysate transduced the ampicillin resistance marker to ~ ~ 5 0 0 6 at a frequency of 5 x 10-l~ per plaque-forming unit adsorbed. Five independently derived transductants were induced with ultraviolet light and y...
متن کاملProperties of Proteus mirabilis and Providence spheroplasts.
Proteus mirabilis strain I 3 and Providence strain NCTC 92 I I were converted to osmotically sensitive spheroplasts by growth in the presence of penicillin or glycine in sucrose-supplemented or unsupplemented medium. Lysazyme in the presence of tris and ethylenediaminetetra-acetic acid (EDTA) converted both these strains to spheroplasts. Penicillin spheroplasts could be stabilized with sucrose,...
متن کاملLipoprotein from Proteus mirabilis.
The biosynthesis of a Proteus mirabilis outer membrane protein of molecular weight of approximately 7,000 was found to be relatively resistant to puromycin and rifampin, as is the case for the Escherichia coli liporotein. Furthermore, the existence of the lipoprotein in P. mirabilis was indicated by a comparison of the amino acid compositions of the purified free and bound forms of this protein...
متن کاملPurification and properties of glucosaminephosphate isomerase of Proteus mirabilis.
A glucosamine-P isomerase has been identified in Proteus mirabilis. The 113-fold purified enzyme exhibits a pH optimum of 7.5 with a secondary maximum at 8.5 and a temperature optimum at 37 degrees C. The apparent Km was 13.3 mM for fructose-6-P and 18.8 mM for L-glutamine. Molecular weight of the enzyme has been estimated as 120 000 and the protein can be dissociated in four subunits by SDS-po...
متن کاملBacteriophage typing of Proteus mirabilis, Proteus vulgaris, and Proteus morganii.
A bacteriphage typing scheme for differentiating Proteus isolated from clinical specimens was developed. Twenty-one distinct patterns of lysis were seen when 15 bacteriophages isolated on 8 Proteus mirabilis, 1 P. vulgaris, and 1 P. morganii were used to type 162 of 189 (85.7%) P. mirabilis and P. vulgaris isolates. Seven phages isolated on 3 P. morganii were used to type 13 of 19 (68.4%) P. mo...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1970
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-9-3-247